BRINE MICROBIOLOGICAL QUALITY USED IN THE PRODUCTION OF FRESH CHEESE IN AN ARTISANAL FACTORY AT Quimiag-Chimborazo

Authors

  • Paola Arguello Escuela Superior Politécnica de Chimborazo, Faculty of Livestock Sciences / Faculty of Public Health / Faculty of Sciences, Food Safety Research and Development Group, Riobamba, Ecuador.
  • Ana Albuja Escuela Superior Politécnica de Chimborazo, Faculty of Livestock Sciences / Faculty of Public Health / Faculty of Sciences, Food Safety Research and Development Group, Riobamba, Ecuador.
  • Ana Rafaela Pacurucu Escuela Superior Politécnica de Chimborazo, Faculty of Livestock Sciences / Faculty of Public Health / Faculty of Sciences, Food Safety Research and Development Group Riobamba, Ecuador.
  • Carlos Pilamunga Escuela Superior Politécnica de Chimborazo, Faculty of Livestock Sciences / Faculty of Public Health / Faculty of Sciences, Food Safety Research and Development Group, Riobamba, Ecuador.

DOI:

https://doi.org/10.47187/perf.v1i24.78

Keywords:

Brine, artisanal cheese, microbiological quality

Abstract

The microbiological quality of the brine used in an artisan cheese factory in the rural parish of Quimiag of the province of Chimborazo, was analyzed, considering that salting process is a critical point during the artisan cheese production. The supplies and materials used for the preparation of the brine, the tools, manipulators and the production environment were sampled from its preparation until the day this solution is discarded. The microbial counts of the new and reused salt exceeded the limits of the Ecuadorian standard for human consumption salt, except for E. coli in the new salt, while S. aureus was identified in the water even after heating received prior use. ATP values measured in tools and manipulators were found between 86.5 to 5279.7 URL, and, the microbial count in the environment exceeded that established by the American Public Health As- sociation of the United States. The level of S. aureus in the brine exceeds the limit of the national regulations for fresh cheeses from the day of its elaboration, and for E. coli and aerobic mesophiles it reaches the maximum allowed on day 5, showing a short shelf life, lack of correct practices of hygiene and the use of contaminated raw materials during brining.

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References

Sousa P. The impact of food manufacturing practices on food borne diseases. Brazilian Archives of biology and Technology. 2008; 51(4): 615-623.

Perin L, Sardaro L, Nero L, Neviani E, Gatti, M. Bacterial ecology of artisanal Minas chee- ses assessed by culture-dependent and-independent methods. Food microbiology. 2017; 65: 160-169.

Irlinger F, Mounier J.Microbial interactions in cheese: implications for cheese quality and safety. Current Opinion in Biotechnology. 2009; 20(2): 142-148.

Arguello P, Escobar S, Espinoza C, Albuja A. “Calidad microbiológica de la materia prima, suero y producto terminado en las queseras artesanales: Quimiag-Ecuador” en: VII Congreso Ibe- roamericano De Ciencias Farmaceuticas Coiffa 2017. Riobamba-Ecuador: COIFFA; 2017. 155-162.

Zheng X, Liu F, Li K, Shi X, Ni Y, Li B, Zhuge B. Evaluating the microbial ecology and meta- bolite profile in Kazak artisanal cheeses from Xinjiang, China. Food research international. 2018;111: 130-136.

Irkin, R. Determination of microbial contamination sources for use in quality management of cheese industry: “Dil” cheese as an example. Journal für Verbraucherschutz und Lebensmittel- sicherheit. 2010; 5(1): 91-96.

Pontín M, Barraza A, Bruschi J. Estudio de la calidad microbiológica y fisicoquímica de salmueras en una quesería [Internet]. Tandil-Argentina. 2017 [citado 28 noviembre 2019]. Disponible en: http://www.ridaa.unicen.edu.ar/xmlui/bitstream/handle/123456789/1472/PONTIN%2C MAXIMILIANO MATIAS.pdf?sequence=1&isAllowed=y.

Salgado M. Efecto del salado directo sobre las características fisicoquímicas, texturales y aceptación de un queso de pasta dura. Universidad Autónoma del Estado de México. 2007; 31 -32.

Norma INEN 1529-2: Control microbiológico de los alimentos. Toma, envío y preparación de muestras para el análisis microbiológico Instituto Ecuatoriano de Normalización (INEN).1999.

Guía técnica sobre criterios y procedimientos para el examen microbiológico de superfi- cies en relación con alimentos y bebidas [Internet]. Perú: Dirección General de Salud Ambiental.2005 [citado 3 diciembre 2019]. Disponible en: http://www.digesa.minsa.gob.pe/norma_consulta/proy_microbiologia.htm

Association of Official Analytical Chemists [Internet]. AOAC Official Method 991.14: Co- liform and Escherichia coli Counts in Foods. AOAC; 1994. [Citado 2 enero 2019]. Disponible en: http://www.longrunbio.com/uploads/soft/130814/1-130Q4144244.pdf.

Silva BO, Caraviello DZ, Rodrigues AC, Ruegg PL. Evaluation of Petrifilm for the isolation of Staphylococcus aureus from milk samples. J Dairy Sci. 2005; 88(8):3000–8

Control de carga microbiana en el aire, método pasivo por sedimentación. [Internet]. Ar- gentina: Instituto Argentino de Normalización y Certificación. 2004 [citado 3 Diciembre2019]. Disponible en: http://catalogo2.iram.org.ar/signven2/busciram01.aspx

Cortizas J, Rumbo J. Nivel de contaminación-descontaminación de la epidermis de las manos medida por luminiscencia. Enfermería Dermatológica. 2015; 9(24):34–45.

Norma INEN 0057: Sal para consumo humano. Requisitos. Instituto Ecuatoriano de Nor- malización (INEN).2010.

Sanchez J. OPS/OMS Peligros biológicos [Internet]. Pan American Health Organization/ World Health Organization. 2015 [citado 3 December 2019]. Disponible en: https://www.paho. org/hq/index.php?option=com_content&view=article&id=10838:2015-peligros-biologicos&Ite- mid=41432&lang=es

Figueroa A, Rivera N, Muñoz J. Implementación del método alternativo petrifilm para de- terminar coliformes y bacterias aerobias mesófilas en la industria de lácteos “Pairumani” y el laboratorio “Lidiveco”. Journal boliviano deficiencias. 2016; 11: 58-65.

Rojas Y, Yesquén G, Vega A, Ascón Y, Vidaurre L, Díaz L. Frecuencia de aislamiento de Staphylococcus aureus Oxacilina resistente en quesos artesanales comercializados en el mercado La Unión (Trujillo, Perú) mayo-julio 2015. REBIOL. 2018; 37(1): 13-18.

Documento de consenso sobre el manejo clínico de las infecciones causadas por Staphylo- coccus aureus resistente a meticilina en adultos [Internet]. Sociedad Andaluza de Enfermedades Infecciosas. 2004 [citado 3 diciembre 2019]. Disponible en: http://www.saei.org/biblioteca/descar- gar/id/28.

Ur Rahman, Shahzad T, Sahar A, Ishaq A, Khan MI, Zahoor T, et al. Recapitulating the competence of novel & rapid monitoring tools for microbial documentation in food systems [In- ternet]. LWT - Food Sci Technol. 2015 [citado 5 noviembre 2019]. Disponible en: http://dx.doi. org/10.1016/j.lwt.2015.11.041.

İpek D, Demirel NN. Microbial load of white cheese process lines after CIP and COP: A case study in Turkey. LWT - Food Sci Technol. 2018; 90:505–12.

Carrascosa C, Millán R, Saavedra P, Jaber JR, Raposo A, Sanjuán E. Identification of the risk factors associated with cheese production to implement the hazard analysis and critical con- trol points (HACCP) system on cheese farms. Journal of Dairy Science. 2016; 99(4): 2606–2616.

Pasquarella C, Pitzurra O, Savino A. The index of microbial air contamination. Journal of Hospital Infection [Internet]. 2000; 46: 241-256. [Citado 6 enero 2019]. Disponible en: https://doi:10.1.1.632.858&rep=rep1&type=pdf

Shelton B, Kirkland K, Flanders W, Morris G. Profiles of airborne fungi in buildings and outdoor environments in the United States. Applied Environmental Microbiology. 2002; 68:1743-1753.

Kragelund M, Johansen P, Harboe A, Castro-Mejía J, Kot W, Krych L, Arneborg N, Jesper- sen L. Cheese brines from Danish dairies reveal a complex microbiota comprising several haloto- lerant bacteria and yeasts. International Journal of Food Microbiology. 2018; 285: 173-187.

Instituto Nacional de Salud de Colombia. Evaluación de riesgos de Staphylococcus aureus enterotoxigénico en alimentos preparados no industriales en Colombia

[Internet]. 2011 [citado 6 junio 2019]. Disponible en: https://www.minsalud.gov.co/sites/rid/Lists/BibliotecaDigital/RIDE/ IA/INS/Erstaphylococcus.pdf.

Albuja A, Andueza L, Escobar S, Yugcha P, Guevara L, Arguello P. Resistencia antimicro- biana de Staphylococcus aureus aislado en quesos frescos artesanales elaborados en zonas rurales de Riobamba-Ecuador. Revista Perfiles. 2018; 20 (2): 76-81.

Gómez J, Medina J, Hochberg D, Mateo-Martí E, Martínez-

Frías J, Rull F. Drying Bacterial Biosaline Patterns Capable of Vital Reanimation upon Rehydration: Novel Hibernating Biomine- ralogical Life Formations. 2014; 14 (7): 589-602.

Norma INEN 1528: Norma General para quesos frescos no madurados. Requisitos. Institu- to Ecuatoriano de Normalización (INEN).2012.

Marino M, Innocente N, Maifreni M, Mounier J, Cobo-Díaz J., Coton, E, et al. Diversity within Italian cheesemaking brine-associated bacterial communities evidenced by massive para- llel 16S rRNA gene tag sequencing. Frontiers in Microbiology.2017; 8: 2119.

Published

2020-08-17

How to Cite

Arguello, P., Albuja, A. ., Rafaela Pacurucu, A. ., & Pilamunga, C. (2020). BRINE MICROBIOLOGICAL QUALITY USED IN THE PRODUCTION OF FRESH CHEESE IN AN ARTISANAL FACTORY AT Quimiag-Chimborazo. Perfiles, 1(24), Faculty of Livestock Sciences / Faculty of Public Health / Faculty of Sciences, Food Safety Research and Development Group. https://doi.org/10.47187/perf.v1i24.78